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	Provedor de dados BJM (2) ABCL (1) Autor Paolicchi,Fernando Alberto (2) Cirone,Karina Mariela (1) Colombo,Daniel (1) Fernández,Bárbara (1) Ferreira,Rachel (1) Fonseca,Leila Souza (1) Gilardoni,Liliana Rosa (1) Gonzaga,Janete da Silva (1) Jar,Ana María (1) Lilenbaum,Walter (1) Marassi,Carla Dray (1) Mendez,Laura (1) Morsella,Claudia (1) Morsella,Claudia Graciela (1) Mundo,Silvia Leonor (1) Oelemann,Walter (1) Ristow,Paula (1) Mais... Palavra-chave Mycobacterium avium subsp. paratuberculosis (3) Cattle (1) Cow's milk (1) Immunomagnetic separation (1) Maduración (1) PCR (1) Serology (1) Viabilidad en quesos (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2016 (1) 2007 (1) 2006 (1) País Brazil (2) Argentina (1) Idioma Inglês (2) Espanhol (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis BJM Marassi,Carla Dray; Gonzaga,Janete da Silva; Ristow,Paula; Ferreira,Rachel; Fonseca,Leila Souza; Oelemann,Walter; Lilenbaum,Walter. An in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (kappa> 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries. Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Mycobacterium avium subsp. paratuberculosis; Serology. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100002 Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR BJM Gilardoni,Liliana Rosa; Fernández,Bárbara; Morsella,Claudia; Mendez,Laura; Jar,Ana María; Paolicchi,Fernando Alberto; Mundo,Silvia Leonor. Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samples from 265 lactating Holstein cows from Map infected and uninfected herds in Argentina. IMS-IS1 PCR results were analyzed and compared with those obtained from milk and fecal culture and serum ELISA. The extent of agreement between both tests was determined by the Kappa test. IMS-IS1 PCR showed a detection limit of 101 CFU of Map/mL of milk, when 50:50 mix of monoclonal and polyclonal antibodies were used to coat magnetic beads. All of the 118 samples... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium avium subsp. paratuberculosis; Immunomagnetic separation; PCR; Cow's milk. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200506 Viabilidad de Mycobacterium avium subsp. paratuberculosis en la maduración de quesos caprinos y bovinos ABCL Cirone,Karina Mariela; Morsella,Claudia Graciela; Colombo,Daniel; Paolicchi,Fernando Alberto. Mycobacterium avium subsp. paratuberculosis (Map) es el microorganismo causante de la paratuberculosis o Enfermedad de Johne, enteritis crónica que afecta a rumiantes y a otros animales domésticos y silvestres. También ha sido vinculado a la enfermedad de Crohn en humanos. Hembras caprinas y bovinas infectadas con Map pueden excretar micobacterias en su leche, que consumida cruda o usada para la elaboración de quesos sin pasteurizar podría constituir una fuente potencial para la infección humana. El objetivo del trabajo fue determinar la viabilidad de Map en quesos elaborados con leche caprina y bovina infectada experimentalmente y sometida o no a tratamiento térmico. Durante la maduración, Map fue aislado sólo en los quesos elaborados con leche sin... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium avium subsp. paratuberculosis; Viabilidad en quesos; Maduración. Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572006000400011 Registros recuperados: 3 Primeira ... 1 ... Última

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